Device

Part:BBa_K2018039:Design

Designed by: Joel Vej-Nielsen   Group: iGEM16_SDU-Denmark   (2016-06-24)


phaCAB genes with additional promoter, that encodes proteins that can create PHB.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 959
    Illegal BglII site found at 1784
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 265
    Illegal NgoMIV site found at 336
    Illegal NgoMIV site found at 936
    Illegal NgoMIV site found at 1248
    Illegal NgoMIV site found at 1527
    Illegal NgoMIV site found at 2179
    Illegal NgoMIV site found at 2201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4045


Design Notes

None


Source

E. coli & R. eutropha Tokyo tech

References